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miniprotean tetra electrophoresis system  (Bio-Rad)


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    Structured Review

    Bio-Rad miniprotean tetra electrophoresis system
    Miniprotean Tetra Electrophoresis System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/miniprotean+tetra/Mini-PROTEAN+Tetra+Electrode+Assembly/pm41995791-98-16-20
    Average 94 stars, based on 123 article reviews
    miniprotean tetra electrophoresis system - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Utilization of self-assembled soy protein nanoparticles as carriers for natural pigments: Examining non-interaction mechanisms and stability
    Article Snippet: In this study, we made self-assembled soy protein nanoparticles (SPN) by partially hydrolyzing soy protein isolate (SPI) using Flavourzyme at different hydrolysis times (0, 0.5, 1, 1.5, 2, 3, and 4 h).. Compared with SPI, SPN (hydrolysis time, 1.5 h) was spherical with smaller particle size, better dispersion, and higher surface hydrophobicity.. The interaction and stability of SPN loaded with different concentrations (0, 0.05, 0.1, 0.3, and 0.5 mmol/L) of lutein (Lut), β-carotene (β-Car), and curcumin (Cur) were then characterized.

    Article Title: Localized release of muscle-generated BDNF regulates the initial formation of postsynaptic apparatus at neuromuscular synapses.
    Article Snippet: Myotomal tissues from Xenopus embryos at Nieuwkoop and Faber stage 19–22 were homogenized in radioimmunoprecipitation assay buffer supplemented with protease inhibitor cocktail and EDTA, and subsequently incubated on ice for 5 min. After high-speed centrifugation at 15,000 × g, the supernatant fraction was collected for protein concentration determination using a BCA protein assay kit (Thermo Fisher Scientific, 23227). .. 30 μg protein lysates were separated by SDS–polyacrylamide gel electrophoresis (SDS–PAGE) using the miniPROTEAN Tetra (Bio-Rad Laboratories), and then transferred to polyvinylidene difluoride (PVDF) membranes using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories). .. After blocking with 3% BSA in Tris-buffered saline with tween 20 (TBST), the blots were probed with the following primary antibodies: anti-BDNF (1:500, abcam, ab108319; RRID: AB_10862052), anti-TrkB (C-13) (1:500, Santa Cruz Biotechnology, sc-119; RRID: AB_632559), MuSK (C-19) (1:1000, Santa Cruz Biotechnology, sc-6009; RRID: AB_2266741), anti-α-tubulin (1:10000; Sigma-Aldrich, T6074; RRID: AB_477582), or anti-β-tubulin (1:1000, DSHB, E7; RRID: AB_528499) at 4 °C overnight.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Utilization of self-assembled soy protein nanoparticles as carriers for natural pigments: Examining non-interaction mechanisms and stability
    Article Snippet: In this study, we made self-assembled soy protein nanoparticles (SPN) by partially hydrolyzing soy protein isolate (SPI) using Flavourzyme at different hydrolysis times (0, 0.5, 1, 1.5, 2, 3, and 4 h).. Compared with SPI, SPN (hydrolysis time, 1.5 h) was spherical with smaller particle size, better dispersion, and higher surface hydrophobicity.. The interaction and stability of SPN loaded with different concentrations (0, 0.05, 0.1, 0.3, and 0.5 mmol/L) of lutein (Lut), β-carotene (β-Car), and curcumin (Cur) were then characterized.

    SDS Page:

    Article Title: Deficiency of muscle-generated brain-derived neurotrophic factor causes inflammatory myopathy through reactive oxygen species-mediated necroptosis and pyroptosis.
    Article Snippet: .. SDS-PAGE was performed using the miniPROTEAN Tetra (Bio-Rad Laboratories, USA), and protein transfer was performed using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories, USA). .. Immunoblotting signals within the linear detection range were detected with a G:Box Chemi XRQ imager (Syngene, UK) and analyzed using ImageJ (NIH, USA).



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